This guideline, mainly for nuclear medicine physicians contains recommendations in terms of procedure, reporting and interpretation in order to standardize Gallium-68 prostate specific membrane antigen (Ga-68 PSMA) positron emission tomography/computed tomography (PET/CT) imaging in prostate cancer (PC). It is thought that guideline recommendations, in which the current scientific place of Ga-68 PSMA PET/CT and developing indications are also discussed, will contribute to increase the accuracy and reliability of Ga-68 PSMA imaging in PC.